| Sunday, Sept 08, 2002 | |
| 14.00-20.00 | Registration |
| 19.00.22.00 | Reception / Dinner / Welcome Address |
| Monday, Sept 09, 2002 | |
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Session 1 - Cell-Matrix interactions Chairperson: Dr Joachim Walter, Boehringer Ingelheim Pharma KG, Germany |
| 08.30-09.00 |
121 Influence of cells on hydrodynamics, thermodynamics, mass
transfer and economics of expanded bed adsorption of proteins. David Curbelo 1 *, Ruben Cuervo 1, Gunnar Garke 2, Wolf-Dieter Deckwer 3, F. Birger Anspach 4 (1) Centre of Molecular Immunology, P.O. Box 16040, Havana 11600, Cuba, (2)Schering AG, Germany, (3) GBF- Gesellschaft für Biotechnologische Forschung mbH, Biochemical Engineering Division, Mascheroder Weg 1, D-38124, Braunschweig, Germany, (4) PHARIS Biotec GmbH, Hannover, Germany |
| 09.00-09.30 |
112 The influence of cell disruption conditions and surface
charge on the Biomass Adsorbent Interactions in Expanded Beds *Peter Brixius1, Dong-Quiang Lin3, Inger Mollerup2, Jürgen Hubbuch1, Maria-Regina Kula1 1 Institut of Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, 52426 Jülich, Germany; 2 Novo Nordisk A/S; Protein Purification, Dept. 427, Hagedornsvej 1, 2820 Gentofte, Denmark, 3 Department of Chemical and Biochemical Engineering, Zhejiang University, 310027, Hangzhou, P. R. China |
| 09.30-10.00 |
103 The Use of Zeta Potential to Characterize Biomass/adsorbent
Electrostatic Interactions in Expanded Bed Dong-Qiang Lin *, Peter Brixius, Jürgen J. Hubbuch, Jörg Thömmes, Maria-Regina Kula, Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, im Forschungszentrum Jülich, 52426 Jülich, Germany |
| 10.00-10.30 | Coffee break |
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Session II - New Matrices Chairperson: Prof. Dr. Howard Chase, University of Cambridge, UK |
| 10.30-11.00 |
123 Design of expanded bed adsorbents towards perfection. Rolf Hjorth, Amersham Biosciences, Uppsala, Sweden |
| 11.00-11.30 |
107 Evaluation of zirconia based ion exchanger for capturing
recombinant endsotain from Pichia pastoris using expanded bed
adsorption. Patrick Santambien1, Egisto Boschetti1, Jim Spenser1, Loc Trinh2 and Joseph Shiloach2*. 1 Biospera, process division of ciphergen biosystems Cergy Saint Christophe, France 2 Biotechnology Unit NIH, NIDDK Bethesda, MD 20892 USA |
| 11.30-12.00 |
120 Optimization of the EBA Performance by the Use of a Robust
High Specific Peptide Ligand Nicole Ameskamp(*), Ronald Frank (1), Norbert Sewald (2), Dirk Lütkemeyer, Jürgen Lehmann Institute of Cell Culture Technology, University of Bielefeld, Germany (1) Institute of Molecular Recognition, GBF Braunschweig, Germany (2) Institute of Organic and Bioorganic Chemistry, University of Bielefeld, Germany |
| 12.15-13.15 | Buffet Lunch |
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Session III - Case Studies Chairperson: Dr. Rhona O’Leary, Genentech Inc, USA |
| 13.30-14.00 |
114 Isolation of Lactoferrin and Other Whey Proteins. Scaling-up
from 1 to 150 cm Diameter Columns having a Rotating Distributing
System. Allan Lihme*, Ulrik Lund Jakobsen, Preben Kønigsfeldt+, Marie Bendix Hansen, UpFront Chromatography A/S, Lersø Parkallé 42 DK-2100 Copenhagen Ø, Denmark, www.upfront-dk.com; +Invensys APV, Pasteursvej, DK-8600 Silkeborg, Denmark. |
| 14.00-14.30 |
131 Overcoming the Difficulties of Performing CEX-Expanded
Bed Chromatography with Low pH E.coli Homogenates Richard L. Gehant* and David W. Kahn Department of Recovery Sciences, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA |
| 14.30-15.00 | To be announced |
| 18.00-19.30 | Poster Session / Refreshments |
| 19.30-22.00 | Dinner |
| Tuesday, Sept 10, 2002 | |
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Session IV - Engineering & Design Chairperson: Mr Rolf Hjort, Amersham Biosciences, Sweden and Dr Jörg Thömmes, University of Düsseldorf, Germany |
| 08.30-09.00 |
119 Scale-Flexible Fluid Distribution System for Expanded
Bed Adsorption Timothy J. Hobley*, Jürgen J. Hubbuch#, Anders Heebøll-Nielsen and Owen R.T. Thomas Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, Søltofts Plads, DK-2800, Kgs. Lyngby, Denmark. #Present address: Institute of Enzyme Technology, Heinrich-Heine University, 52426 Jülich, Germany. |
| 09.00-09.30 |
132 THE EFFECT OF FLUID INLET DESIGNS ON MAMMALIAN CELL CULTURE
VIABILITY Jan Feuser*, Barbara Dietl, Daniel Buchholz, Ann-Kristin Barnfield Frej1, Mats Lundkvist1, Joachim K Walter Boehringer Ingelheim Pharma KG, Biberach, Germany, 1Amersham Biosciences, Uppsala, Sweden |
| 09.30-10.00 |
102 Development of Buoyancy-Induced Mixing in Expanded Beds Conan J. Fee*, Allan Neilsen, Jon Petersen, Michael Walmsley, Jens Bjerkwig, Lars Schultz, Dept of Materials & Process Engineering, University of Waikato, Hamilton, New Zealand |
| 10.00-10.30 | Break |
| 10.30-11.00 |
111 Modeling the Combined Effects of Particle Size Distribution and Process Operational Parameters on the Performance of Expanded Bed Adsorption Process Magnus Wistranda,b and Karol Lacki a* a Amersham Biosciences AB, Uppsala, Sweden; b Department of Molecular Biotechnology, Uppsala University, Uppsala, Sweden |
| 11.00-11.30 | To be announced |
| 11.30-12.00 | To be announced |
| 12.15-13.15 | Buffet Lunch / Box Lunch |
| 13.15-17.30 | Social Program |
| 17.30-19.30 | Poster Session / Refreshments |
| 19.30-22.00 | Dinner |
| Wednesday, Sept 11, 2002 | |
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Session V - Novel Applications Chairperson: Prof. Dr. Maria-Regina Kula, University of Düsseldorf, Germany |
| 08.30-09.00 |
109 A novel process for continuous protein refolding and on-line
EBA capture Henrik Ferré*1, Owen R.T. Thomas1, Timothy J. Hobley1, Søren Buus2 1 Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, Søltofts plads, DK-2800, Kgs. Lyngby, Denmark 2 Institute of Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen, Denmark |
| 09.00-09.30 |
106 Application of Expanded Bed Adsorption Chromatography
to Protein Recovery from Fusion Protein Cleavage C. W. Suh, S. J. Nah, and E. K. Lee, Bioprocessing Research Laboratory Department of Chemical Engineering, Hanyang University, Ansan, KOREA 425-791 |
| 09.30-10.00 | To be announced |
| 10.00-10.30 | Break |
| 10.30-11.00 |
116 EBA processing of potato fruit water on mixed mode adsorbent
for functional protein recovery: A difficult separation task
made possible. Knut O. Strætkvern *, Hedmark College, Dept. of Agriculture and Natural Sciences, N-2322 Ridabu, Morten Aae Olander and Allan Lihme, UpFront Chromatography A/S, DK-2100 Copenhagen, Denmark |
| 11.00-11.30 |
124 Cell Separations in Expanded Beds Layth Ujam, Robert Clemmitt & Howard Chase* Department of Chemical Engineering, University of Cambridge Pembroke Street, Cambridge CB2 3RA, U.K. |
| 11.30-12.00 | To be announced |
| 12.00-12.30 | Closing Remarks and Adjourn / Box Lunch |